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. Author manuscript; available in PMC: 2022 Sep 22.
Published in final edited form as: Cell Rep. 2022 Sep 6;40(10):111306. doi: 10.1016/j.celrep.2022.111306

Figure 3. Mesenteric arteries from fat-1 mice display enhanced TRPV4-mediated vasodilation.

Figure 3.

(A) Representative time course of GSK101 (5 nM)-induced vasodilation of pressurized (80 mmHg) mesenteric arteries from WT and fat-1 mice. Inset: micrograph of a representative cannulated mesenteric artery.

(B) Percentage of GSK101 (5 nM)-induced vasodilation of mesenteric arteries (endothelium-intact or -denuded) from WT and fat-1 mice. Bars are mean ± SEM. Two-way ANOVA and Tukey multiple comparisons test.

(C) Representative western blot (anti-TRPV4) of the membrane fractions of WT and fat-1 mice mesenteric arteries.

(D) Mean/scatter-dot plot showing relative intensities of TRPV4 bands, calculated from total protein detection of chemically labeled proteins (Stain-Free System Bio-Rad), from the membrane fractions of mesenteric arteries from WT and fat-1 mice. Lines are mean ± SD. Two-tailed unpaired t test. Asterisks indicate values significantly different from WT (**p < 0.01) and n.s. indicates values not significantly different from the WT. n is indicated in each panel. See also Figure S3.