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. Author manuscript; available in PMC: 2022 Sep 22.
Published in final edited form as: Cell Rep. 2022 Sep 6;40(10):111306. doi: 10.1016/j.celrep.2022.111306

Figure 7. TRPV4 proximal N terminus determines the effect of EPA on desensitization.

Figure 7.

(A) Cartoon depicting the full-length rat TRPV4 channel.

(B) Representative time course of whole-cell patch-clamp recordings (−60 mV) of control and EPA (100 μM)-treated HEK293 cells, expressing full-length rat TRPV4, challenged with GSK101 (1 μM). Traces were normalized for comparison.

(C) Boxplots show mean (gray circle), median (bisecting line), SD (whiskers), and SEM (box) of the time to reach half amplitude from the peak current (Imax) elicited by GSK101 (1 μM) in control and EPA (100 μM)-treated HEK293 cells expressing full-length rat TRPV4. Two-tailed unpaired with Welch’s correction.

(D) Cartoon depicting the Δ186 rat TRPV4 channel construct.

(E) Representative time course of whole-cell patch-clamp recordings (−60 mV) of control and EPA (100 μM)-treated HEK293 cells, expressing the Δ186 rat TRPV4 channel construct, challenged with GSK101 (1 μM). Traces were normalized for comparison.

(F) Boxplots show mean (gray circle), median (bisecting line), SD (whiskers), and SEM (box) of the time to reach half amplitude from the peak current (Imax) elicited by GSK101 (1 μM) in control and EPA (100 μM)-treated HEK293 cells expressing the Δ186 rat TRPV4 channel construct. Two-tailed unpaired t test.

(G) Cartoon depicting the 5Ala (K121A, R122A, R124A, R125A, K126A) rat TRPV4 channel construct.

(H) Representative time course of whole-cell patch-clamp recordings (−60 mV) in control and EPA (100 μM)-treated HEK293 cells expressing the 5Ala rat TRPV4 channel construct challenged with GSK101 (1 μM). Traces were normalized for comparison.

(I) Boxplots show mean (gray circle), median (bisecting line), SD (whiskers), and SEM (box) of the time to reach half amplitude from the peak current (Imax) elicited by GSK101 (1 μM) of control and EPA (100 μM)-treated HEK293 cells expressing the 5Ala rat TRPV4 channel construct. Two-tailed unpaired t test. Asterisks indicate values significantly different from control (**p < 0.01) and n.s. indicates values not significantly different from the control. n is indicated in each panel. See also Figure S5.