TABLE 3.
IncP/P-1 plasmids obtained in this study
| Name | Inc_group | Size (bp) | Source | Antibiotic resistance genes and the resistances of the hosts (R, resistance, S, sensitive, ND, not determined)a |
||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Accessory genes | Tc (12.5)b | Gm (30) | Ap (50) | Km (50) | Km (25) | Km (12.5) | Cm (30) | Sm (50) | Sm (25) | Em (25) | ||||
| pYKBP039 | IncP/P-1β-2 | 55,596 | Activated sludge | Tn402-class1 integron (blaOXA, blaGES, aac(6′)-Ib, ere(A), blaIMP, aac(6′)-Ib) | ND | S | R | S | R | R | ND | ND | ND | R |
| pYKBO007 | IncP/P-1ε-I | 42,530 | Activated sludge | Transposon | ND | ND | ND | ND | ND | ND | ND | ND | ND | ND |
| pYKBR041 | IncP/P-1ι | 45,846 | Activated sludge | aac(6′)-31 | ND | ND | ND | R | R | R | ND | ND | ND | ND |
| pYKCG107 | IncP/P-1ι | 46,366 | Activated sludge | Transposon | ND | ND | ND | ND | ND | ND | ND | ND | ND | ND |
| pMNCG080-1 | IncP/P-1ρ | 69,454 | Activated sludge | No accessory | ND | ND | ND | ND | ND | ND | ND | ND | ND | ND |
| pMNCG082-1 | IncP/P-1μ | 74,115 | Activated sludge | bcs | ND | ND | ND | ND | ND | ND | ND | ND | ND | ND |
| pSM0227-07 | IncP/P-1β-1 | 51,612 | Anaerobic WWTP | Tn402 with mgtAC | ND | ND | ND | ND | ND | ND | ND | ND | ND | ND |
| pSM0227-02 | IncP/P-1δ | 47,983 | Anaerobic WWTP | dam, alwI | ND | ND | ND | ND | ND | ND | ND | ND | ND | ND |
| pMH0621-02Tc | IncP/P-1β-1 | 64,795 | Lake sediment | sul2, tet(X6), tetG mer operon, Tn21-like transposon | R | ND | ND | ND | ND | ND | ND | ND | ND | ND |
| pMHAD031 | IncP/P-1θ | 42,885 | Lake sediment | No accessory | ND | ND | ND | ND | ND | ND | ND | ND | ND | ND |
| pMNCN061 | IncP/P-1α | 64,346 | Marine sediment | tetAR, strAB, blaSHV, aph(3′)-Ib | R | S | R | ND | ND | ND | ND | S | R | ND |
| pMNCN064 | IncP/P-1α | 68,925 | Marine sediment | tetAR, transposon (Tn402-class1 integron [qacH, aadA1, sul1], mer operon), aph(3′)-Ib | R | S | ND | R | R | R | ND | S | S | ND |
| pYKBF005 | IncP/P-1β-1 | 60,124 | River sediment | Tn402-class1 integron (qacEΔ-sul1-orf5, aac[6′]-IIa, aadA6, blaOXA), Tn (relE) | ND | S | R | S | S | R | ND | S | S | ND |
| pYKBU009 | IncP/P-1β-1 | 53,186 | River sediment | Tn501(mer operon), IS1071 | ND | ND | ND | ND | ND | ND | ND | ND | ND | ND |
| pYKCT011-1 | IncP/P-1β-1 | 57,620 | River sediment | Tn501(remnant) (mer operon), IS21, Tn3 family (blaNPS), relE | ND | ND | R | ND | ND | ND | ND | ND | ND | ND |
| pMNCI060 | IncP/P-1β-1 | 56,363 | River sediment | Transposon (aph[3′]-Ia, tetCR, strAB), tetAR | R | S | R | ND | ND | ND | ND | R | R | ND |
| pMNCI062 | IncP/P-1β-1 | 38,628 | River sediment | No accessory | ND | ND | ND | ND | ND | ND | ND | ND | ND | ND |
| pMNCI063 | IncP/P-1β-1 | 52,178 | River sediment | aroA | ND | ND | ND | ND | ND | ND | ND | ND | ND | ND |
| pYKCS045 | IncP/P-1γ | 49,370 | River sediment | Tn402-class1 integron (like) (blaGES-5, aac[6′]-Ib) | ND | S | R | S | S | R | ND | ND | ND | ND |
| pMNBL056 | IncP/P-1ε-I | 52,432 | River sediment | Class1 integron (dfrB1, qacEΔ-sul1-orf5, tetRA) | R | S | S | ND | ND | ND | ND | ND | ND | ND |
| pYKBL037 | IncP/P-1ι | 64,506 | River sediment | Transposon (strAB, class1 integron(aac(6′)-Ib, ere(A), qacEΔ-sul1-orf5). Transposon (blaAER, qacL) | ND | S | R | R | R | R | ND | S | R | R |
| pMNBM077 | IncP/P-1κ | 53,339 | River sediment | Transposon (tetAR, transposon) | R | S | S | ND | ND | ND | ND | ND | ND | ND |
| pYKAZ004 | IncP/P-1β-1 | 58,771 | Soil | Tn402-class1 integron (qacEΔ-sul1-orf5), IS1071 | ND | ND | ND | ND | ND | ND | ND | ND | ND | ND |
| pYKBG006 | IncP/P-1β-2 | 51,488 | Soil | IS1071 | ND | ND | ND | ND | ND | ND | ND | ND | ND | ND |
| pYKAM033 | IncP/P-1β-2 | 42,722 | Soil | No accessory | ND | ND | ND | ND | ND | ND | ND | ND | ND | ND |
| pYKBG036 | IncP/P-1ο | 43,728 | Soil | ompA | ND | ND | ND | ND | ND | ND | ND | ND | ND | ND |
| pYKAM101 | IncP/P-1λ | 69,067 | Soil | Two ISs | ND | ND | ND | ND | ND | ND | ND | ND | ND | ND |
a
Tc, tetracycline; Gm, gentamicin; Ap, ampicillin; Km, kanamycin; Cm, chloramphenicol; Sm, streptomycin; Em, erythromycin.
b
The digits in parentheses indicate the concentration of each antibiotic (μg/mL).