TABLE 5.

Distribution of IncP/P-1 and PromA plasmids in different environmental samples^a

Environmental samples	IncP/P-1b										PromA
	Clade I					- - -			Clade II		-
	αβε	ι	δ	θ	γ	κ	ο	λ	η	ρμ	αβγδ
Activated sludge (4WWTPs, 20 samples)	11(14)	8(2)	1(0)	0(0)	1(0)	0(0)	0(0)	ND(0)	0(0)	1(2)	5(6)
Anaerobic WWTP (2 samples)	ND(1)	ND(0)	ND(1)	ND(0)	ND(0)	ND(0)	ND(0)	ND(0)	ND(0)	ND(0)	ND(1)
Cow manure (1 sample)	0(0)	0(0)	0(0)	0(0)	0(0)	0(0)	0(0)	ND(0)	0(0)	0(0)	1(0)
Lake sediment (17 lakes, 21 samples)	0(3)	0(0)	0(21)	0(1)	0(1)	0(0)	0(0)	ND(0)	0(0)	0(0)	7(67)
Marine sediment (2 sites, 10 samples)	0(2)	0(0)	0(0)	0(0)	0(0)	0(0)	0(0)	ND(0)	0(0)	0(0)	0(0)
Paddy sediment (1 sample)	0(0)	0(0)	0(0)	0(0)	0(0)	0(0)	0(0)	ND(0)	0(0)	0(0)	1(0)
Pond sediment (3 samples)	0(4)	0(0)	0(8)	0(0)	0(0)	0(0)	0(0)	ND(0)	0(0)	1(0)	1(0)
River sediment (9 rivers, 40 samples)	0(7)	0(1)	2(11)	0(0)	0(1)	0(1)	0(0)	ND(0)	0(0)	2(0)	6(39)
Soil (10 sites, 17 samples)	1(19)	0(0)	1(9)	0(0)	1(0)	0(0)	0(1)	ND(1)	0(0)	0(0)	3(2)
Total (47 sites, 113 samples)	12 (50)	8(3)	4(50)	0(1)	2(2)	0(1)	0(1)	0(1)	0(0)	4(2)	24(115)

^aThe digits indicate the number of environmental samples showing positive signals with the specific probe in PCR-Southern blot analyses, whereas those in parentheses indicate the number of isolates with the plasmid (Tables S1 and S2). ND, not determined.

^bNewly proposed subgroups of IncP/P-1 group are shown in bold. -, indicates IncP/P-1 plasmids not included by clades I or II.