TABLE 1.

The primers used in this study

Primer	Sequence (5′–3′)	Usage
2999ColdF	gcggtggcggtagcggtATGTGCACTAAATCCAAAG	Cloning gene 2999 to ligate into linearized pCold-SUMO
2999ColdR	GGTGGTGGTGGTGCTCGAGTGCGGCCGCAAGCTTTTTTTATGCCAAACTCTTAAATATTC
3002ColdF	gcggtggcggtagcggtATGCAGAAGGAGAAGGC	Cloning gene 3002 to ligate into linearized pCold-SUMO
3002ColdR	GTGGTGGTGGTGCTCGAGTGCGGCCGCAAGCTTATATTATTCTTTTGCCAAACGC
ph1656F	ggcggtggcggtagcggtGAAGTTCTGCCGCTGAGCG	Cloning gene ph1656 to ligate into linearized pCold-SUMO
Ph1656R	GGTGGTGCTCGAGTGCGGCTTTCTGCCACACACGC
SUMO-2999/3002F	CCGCACTCGAGCACCACCACCACCACCACTGAaagcttgtcgacctgcag	To linearize the plasmid pCold-SUMO without SUMO domain for linking 2999 and 3002
SUMO-2999/3002R	aatgggtcgcggatccGCAAATGGAACTCCACC
2959ColdF	cggtaccctcgagggatccATGTATGCGATCTTGACATC	Cloning gene 2959 to ligate into linearized pCold-SUMO
2959ColdR	agtgcggccgcaagcttTTTTTTTATGATTTTTATAGCATATTTTTTTTC
2991ColdF	gtaccctcgagggatccATGATAGGGACAAGCTTC	Cloning gene 2991 to ligate into linearized pCold-SUMO
2991ColdR	ctgcaggtcgacaagcttCTTTTTATCCTTTAACTCCAC
ColdF	aagcttgtcgacctgcagt	To linearize the plasmid pCold-SUMO for linking 2959 and 2991
ColdR	ggatccctcgagggtaccg