FIG 4.

RecT impacts the excision frequency of PP9W rather than the relative adsorption rate. (A) Excision frequency analysis in different strains. Each sample was tested in sextuplicate. (B) LPS content quantification in different groups after adsorption with related phages. Each sample was tested in quintuplicate. (C) Adsorption rate assay for PP9W (black line) and PP9D (red line) on P8W. The plasmid pCT2 carries the recT gene. P8W/pCT2+PP9D represents the complementation group (green line). (D) Evaluation of the impacts of recT on the excised phages in the supernatant of P9W. The black border bar represents the bacterial biomass of different strains after growth for 12 h. The red border bar shows the corresponding number of free phages in the supernatant. The experiments were independently replicated three times (A to D), and each sample was tested in triplicate (C and D). Error bars show standard deviations. Data were analyzed by one-way analysis of variance (ANOVA) with Tukey’s multiple comparisons (α < 0.05) to examine the mean differences between the data groups. **, P < 0.01; ns, no significance.