TABLE 1.
Bacterial strains, phages, and plasmids used in this study
| Category | Genotype or description | Source |
|---|---|---|
| P. aeruginosa | ||
| P7 | Wild type P. aeruginosa clinical isolate | This work |
| P8W | Wild type P. aeruginosa clinical isolate | This work |
| P9W | Wild type P. aeruginosa clinical isolate | This work |
| P18 | Wild type P. aeruginosa clinical isolate | This work |
| P27 | Wild type P. aeruginosa clinical isolate | This work |
| P9D | P9W ΔrecT | This work |
| E. coli | ||
| DH5α | F− ϕ80 lacZΔM15 Δ(lacZYA-argF) U169 endA1 recA1 endA1 hsdR17(rK−, mK+) supE44λ− thi-1 gyrA96 relA1 phoA | TianGen |
| S17-1 | RP4-2 Tc::Mu Km::Tn7 Tpr Smr Pro Res− Mod+ | TransGen |
| JM109 | endA1 recA1 gyrA96 thi hsdR17(rk−, mk+) relA1 supE44D(lac-proAB) [F’traD36 proAB laq lqZΔM15] | Biomed |
| BL21(DE3) | F-ompT hsdSB(rB−, mB−) gal dcm(DE3) | Biomed |
| Phages | ||
| PP9W | A excised prophage isolated from P9W | This work |
| PP9W2 | Another excised prophage isolated from P9W | This work |
| PP9D | The recT gene deletion derivative of PP9W | This work |
| Plasmids | ||
| pEX18Tc | Gene replacement vector; Tcr oriT+ sacB+ | W. Wu |
| pEX18Tc::U + D | The recT gene knockout vector | This work |
| pUCP18 | Broad host range shuttle vector, Apr | W. Wu |
| pCT2 | The recT gene complementation vector | This work |
| pUC19 | Commonly used cloning vector; lac lacZ Apr | NEB |
| pMD19 (Simple) | A linearized T-vector for cloning promoter fragments, Apr | TaKaRa |
| pEDS40 | pg40 gene promoter cloned in pMD19 (Simple), Apr | This work |
| pEDS50 | pg50 gene promoter cloned in pMD19 (Simple), Apr | This work |
| pEDS55 | pg55 gene promoter cloned in pMD19 (Simple), Apr | This work |
| pEDS57 | pg57 gene promoter cloned in pMD19 (Simple), Apr | This work |
| pEDS72 | pg72 gene (IntR) promoter cloned in pMD19 (Simple), Apr | This work |
| pET32a(+) | Fusion vector for N-terminal His tag, Apr | NovaGen |
| pET32a-T | his-recT fusion in pET32a(+) vector, Apr | This work |
| pEASY-T1 | Cloning vector for ploting the standard curves, Apr Kanr | TransGen |
| pEASY-I | T-I fusion in pEASY-T1 vector, Apr Kanr | This work |
| pEASY-A | T-pg47 fusion in pEASY-T1 vector, Apr Kanr | This work |