Table 1.
HDR efficiency and mutation types for the OsLcyE gene using the CRISPR-Cas9-based geminiviral replicon system.
Targeted Region | No. of Examined Calli | DNA Repair Pathway | No. of Edited Calli (%) | Mutant Type | No. of Mutation |
---|---|---|---|---|---|
LcyE-sg1 | 83 | NHEJ | 48 (57.8%) | Homo | 8 |
Bi-allelic | 13 | ||||
Hetero | 27 | ||||
HDR | 0 (0%) | - | - | ||
LcyE-sg2 | 304 | NHEJ | 169 (54.5%) | Homo | 24 |
Bi-allelic | 46 | ||||
Hetero | 93 | ||||
HDR | 4 (1.32%) | Homo | 4 |