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(A) Schematic show CTCF knockdown prevents TAD formation during human ZGA. (B) Venn diagram shows the overlap between predicted constitutive CTCF binding sites in 2-cell and 8-cell embryos. (C) Metagene plots show aggregated cleavage profiles of predicted 2-cell specific (Group A), common (Group B) and 8-cell specific (Group C) in 2-cell and 8-cell embryos.
