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. 2022 Sep 12;18(9):e1010811. doi: 10.1371/journal.ppat.1010811

Fig 6. SIRT5-KO cells mount a stronger innate immune response.

Fig 6

RNA-seq analysis of WT and SIRT5-KO A549-ACE2 cells infected or mock-infected for 3 days with SARS-CoV-2 at MOI = 1. n = 4. A. Volcano plots showing differentially expressed genes between the different conditions. Highlighted genes display a q-value q<0.05 (green), log2 fold-change >1 (orange), or both (purple). Left panel: SIRT5-KO vs WT in mock-infected cells. Middle: Infected vs mock-infected WT cells. Right: Infected vs mock-infected SIRT5-KO cells. B. Normalized gene count of SARS-CoV-2. C-D. Unsupervised clustering of the 3221 genes differentially expressed between at least two of the four conditions (q<0.01). C: heatmap of normalized expression. D. Z-scores of differentially expressed genes as grouped by clustering. Colored lines represent the quantification of an individual gene whereas solid black lines show the cluster Tukey boxplot. E. Enrichment analysis of biological gene sets in the identified gene clusters (C and D).