A. Expression heatmap of interferon-stimulated genes and other restriction factors, showing that mock-infected SIRT5-KO cells express higher basal levels of restriction factors, and that antiviral responses are stronger in SIRT5-KO cells. Data show mean log2 fold-change, compared to mock-infected WT, and the q-value between mock-infected WT and SIRT5-KO cells. Only genes differentially expressed between at least two conditions were included in the analysis (q<0.01). B. RT-qPCR confirmation of restriction factors upregulated in non-infected SIRT5-KO cells (n = 8). Data show fold-changes compared to WT levels after normalization with ACTIN. Data show mean and SEM between replicates. p-values after unpaired two-tailed t-test are shown and asterisks summarize the results. *: p < 0.05, **: p < 0.01, ***: p < 0.001, ****: p < 0.0001.