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. 2022 Sep 22;17(9):e0270436. doi: 10.1371/journal.pone.0270436

Table 1. Female longevity responses of four Drosophila species in response to four dietary treatments.

Species and treatment n mean SEM p-value median range skew kurtosis
D. mel.
    no yeast 26 19.12 1.01 0.0001 19.50 27 -1.24 3.97
    Minimum 27 28.44 2.92 - 28.00 59 0.61 0.47
    ad libitum 30 23.37 3.12 >0.05 23.00 66 0.66 0.19
    ad libitum with rapa 31 12.13 1.14 1.1x10-7 12.00 25 0.06 -0.60
D. gut.
    no yeast 30 12.40 1.03 6.8x10-7 14.50 18 -0.46 -1.08
    Minimum 30 30.30 3.76 - 25.00 74 0.85 0.03
    ad libitum 30 55.60 5.60 3.5x10-4 51.00 124 0.48 0.41
    ad libitum with rapa 30 28.03 3.95 >0.5 17.00 76 0.81 -0.50
D. def.
    no yeast 29 8.17 0.35 5.1x10-9 8.00 9 -0.20 1.40
    Minimum 30 16.87 1.38 - 16.00 30 0.36 -0.58
    ad libitum 30 36.43 3.68 2.9x10-7 34.50 75 0.08 -0.68
    ad libitum with rapa 30 27.47 2.47 3.7x10-5 27.00 53 -0.17 -0.13
D. tri.
    no yeast 26 13.19 0.62 8.6x10-7 13.50 15 -2.36 8.17
    Minimum 29 32.48 4.36 - 25.00 89 1.25 0.85
    ad libitum 29 60.31 5.33 1.6x10-4 57.00 118 0.08 -0.17
    ad libitum with rapa 29 68.24 4.17 4.5x10-6 75.00 71 -0.38 -1.09

Measures of female lifespan in [days] (mean and median) for D. mel., D. gut., D. def., and D. tri. treated with diets ranging from no yeast, minimum (1 scoop/fly/day), to ad libitum, as well as an ad libitum yeast diet on rapamycin-treated medium (200 μM). n = number of flies going into the analysis; escaped or flies killed by accident were discarded. The mean and median values are given in [days]. p-Values were obtained by comparing test conditions to minimum feeding of that species and analyzed using pairwise comparison using Log-Rank.