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(a) Yeast one-hybrid assays; (b) Identification of TaWRKY22 binds to W-box of TaCOPT3D promoter. (I): Arabidopsis expression of TaCOPT3D-GUS; (II): Arabidopsis expression of TaWRKY22; (III): Arabidopsis co-expression of TaCOPT3D-GUS and TaWRKY22; (c) EMSA assay showing that TaWRKY22 fusion protein directly bound to the TaCOPT3D promoter in vitro; (d) Transient expression assays showing that TaWRKY22 regulates the expression of TaCOPT3D.
