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. 2022 Sep 8;23(18):10400. doi: 10.3390/ijms231810400

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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miR-486-5p downregulated Smad2 and Smad3 in HPASMCs. (A) HPASMCs were transfected with the miR-486-5p mimic or NC mimic, and the total mRNA and cell lysate were collected after 48 h. The protein expression and phosphorylation of Smad2 and Smad3 were determined with Western blots. (B) The intensities of p-Smad2, p-Smad3, Smad2, Smad3, and β-actin were quantified using the ImageJ software. (C) The mRNA level of Smad2 was determined by qRT-PCR. Results are expressed as the means ± SEMs (n = 4). * p < 0.05 compared with the NC mimic group.