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. 2022 Sep 7;23(18):10319. doi: 10.3390/ijms231810319

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Compound (1s) decreases HIF-dependent gene transcription. MDA-MB-468-UnaG cells were pre-treated for 1 h without (CTRL) or with compound (1s) or BAY 11-7085 (BAY) before being grown (a,b) in the absence (normoxia; CTRL) or (c,d) presence of 100 µM CoCl₂ (hypoxia) for another 24 h. (a,c) Representative fluorescence images. Scale bar: 200 µm (b,d) Total fluorescence intensity of HIF-mediated UnaG was normalized to the CTRL group. Statistical difference was calculated with one-way ANOVA (b) vs. CTRL (normoxia) (ns = not significant, * p < 0.05). (d) vs. CTRL (hypoxia) (# p < 0.05); vs. CoCl₂ (* p < 0.05, ** p < 0.01, *** p < 0.001). (b,d) Columns represent the mean ± SEM (n = 3).

Compound (1s) decreases HIF-dependent gene transcription. MDA-MB-468-UnaG cells were pre-treated for 1 h without (CTRL) or with compound (1s) or BAY 11-7085 (BAY) before being grown (a,b) in the absence (normoxia; CTRL) or (c,d) presence of 100 µM CoCl₂ (hypoxia) for another 24 h. (a,c) Representative fluorescence images. Scale bar: 200 µm (b,d) Total fluorescence intensity of HIF-mediated UnaG was normalized to the CTRL group. Statistical difference was calculated with one-way ANOVA (b) vs. CTRL (normoxia) (ns = not significant, * p < 0.05). (d) vs. CTRL (hypoxia) (# p < 0.05); vs. CoCl₂ (* p < 0.05, ** p < 0.01, *** p < 0.001). (b,d) Columns represent the mean ± SEM (n = 3).