Figure 8.
Compound (1s) stabilizes IκBα under normoxic and under hypoxic conditions. (a,c) For hypoxia studies, MDA-MB-468-UnaG cells were exposed to 100 µM CoCl2 for 24 h prior to treatment. (a–c) Cells were left untreated (CTRL), stimulated with solely 10 ng/mL TNFα for 30 min (TNFα), or pre-incubated with 20 nM of compound (1s) for 1 h before stimulation with TNFα for 30 min. (a) Normalized IκBα protein level is displayed relative to CTRL. Statistical difference vs. TNFα was calculated with two-way ANOVA (* p < 0.05, ** p < 0.01, *** p < 0.001). Columns represent the mean ± SD of (normoxia n = 3; hypoxia n = 2). (b,c) Representative western blot with protein lysates that are immunologically stained for IκBα and the housekeeper protein vinculin.