Figure 3.

Inhibitory effect of StpA on bgl operon expression in the absence of H-NS. Using the operon reporter, Pbgl-bglG-lacZ (at the lac locus), the bgl operon transcriptional activities were assayed, comparing the stpA single mutant (∆stpA), the hns single mutant (∆hns), and the hns/stpA double mutant (∆hns∆stpA). Cells were cultured in M63 with glycerol and salicin. Sample preparation and β-galactosidase assays were carried out as in Figure 1.