Skip to main content
. 2022 Sep 7;23(18):10343. doi: 10.3390/ijms231810343

Figure 5.

Figure 5

Fis represses bgl operon expression when it is activated by IS insertion. (A) Fis repression of bgl operon expression after activation by IS insertion. (B) Antagonism between Fis and Crp in regulating the bgl operon. In both (A) and (B), IS5 insertion is present both in the native operon and the operon lacZ reporter. Cells were cultured in M63 with glycerol and salicin as carbon sources. Sample collection and β-galactosidase assays were carried out as in Figure 1.