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. 2022 Sep 6;23(18):10242. doi: 10.3390/ijms231810242

Figure 5.

Figure 5

The targeting of TIM-1 by miR-142 significantly reduced endothelial permeability. Endothelial leakage triggered by 48 h incubation with TIM-1 agonist TIMD4 (10 or 100 ng/mL; BioLegend San Diego, CA, USA) was measured in hBMECs transfected with miR-142 mimic, miR scramble, or by combining miR-142 mimic and TIM-1 overexpression (pcDNA3.1-TIM-1 plasmid; GenScript, Piscataway, NJ, USA). All experiments were performed at least in triplicate; in the violin plot, median (solid line) and quartiles (dotted lines) are indicated; * p < 0.01 vs. miR scramble, # p < 0.01 vs. vehicle.