Figure 2.

The SCD deficiency affects the HIF1α and VEGF-A expression in post-MI hearts. (A) Protein levels of HIF1α and VEGF-A in the sham and post-MI myocardium in the WT, SCD1^−/−, and SCD4^−/− mice determined by Western blot. The data are representative of n = 5 mice/group. The data are expressed as the mean ± SD. * p < 0.05, vs. WT sham; ^# p < 0.05, vs. SCD1^−/− or SCD4^−/− sham; ^& p < 0.05, vs. SCD1^−/− or SCD4^−/− sham (two-sided t-test). (B) The HL-1 cells treated with 2 µM A939572 (SCD1 inhibitor) were subjected to hypoxia for 18 h. The VEGF-A concentration in culture medium was measured by ELISA. The data are representative of n = 3 independent experiments. The data are expressed as the mean ± SD. a—p < 0.05, vs. control/normoxia; c—p < 0.05, vs. A939572/normoxia; e—p < 0.05, vs. control/hypoxia (one-way analysis of variance [ANOVA]).