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. 2022 Sep 9;23(18):10459. doi: 10.3390/ijms231810459

Figure 4.

Figure 4

SCD1 deficiency/inhibition affects new vessel formation. (A) SCD1 inhibition affects the angiogenic potential of hypoxic HL-1 cardiomyocytes. Human EA.hy926 endothelial cells were cultured in conditioned medium from HL-1 cardiomyocytes that were treated with 2 µM A939572 (SCD1 inhibitor) and/or subjected to hypoxia for 18 h. The number of nodes and meshes and length of tube-like structures were measured using Angiogenesis Analyzer for ImageJ software. The data are representative of n = 3 independent experiments. The data are expressed as the mean ± SD. a—p < 0.05, vs. control/normoxia; c—p < 0.05, vs. A939572/normoxia; e—p < 0.05, vs. control/hypoxia (ANOVA). (B) Effect of HF diet and/or SCD1 deficiency on the angiogenic potential of aortic explants. The ex vivo ability of aorta rings to sprout blood vessel-like capillary structures was demonstrated. Photomicrographs were captured 4 days after embedding aortic rings in the collagen matrix. The number of junctions and segments and branches were analyzed using Angiogenesis Analyzer for ImageJ software. The data are representative of n = 3–5 independent experiments. The data are expressed as the mean ± SD. * p < 0.05, vs. WT chow; & p < 0.05, vs. WT HF (ANOVA).