FIG. 1.

Map of the pnb gene cluster of TW3. Locations of the five open reading frames are marked by open arrows, the direction of the arrowheads indicating the direction of transcription. The inserts of recombinant plasmids are represented below. pTW3.11 and pTW3.13 were subcloned from a large cosmid clone, and pTW3.12, pTW3.14, and pTW3.15 were derived from them by further subcloning. Only restriction sites relevant to the clones and constructs described in this paper are shown. The symbols at the right indicate which plasmids in E. coli accumulated PCA in media containing p-toluidine and 4NBen, and which were able to confer on PaW340 the ability to grow on 4NBen (ND, not determined).