TABLE 3.
Specific activities and stoichiometry of 4NBen reductase
| Source of cell extract | Growth or induction | Sp act (mU/mg of protein) | Stoichiometry of NADH oxidation (mol of NADH oxidized/mol of 4NBen utilized) |
|---|---|---|---|
| P. putida TW3 | 4NBen | 260 | 1.79 |
| Succinate | <10 | NDa | |
| E. colib(pTW3.15) | Uninduced | 230 | 1.85 |
| E. colib(pTW3.15NH) | Uninduced | 590 | 1.87 |
| E. colib(pTW3.15NX) | Uninduced | <10 | ND |
| E. colib(pUC18) | Induced | <10 | ND |
| E. colic(pPROpnbA) | Induced | 400 | 1.84 |
| E. colic(pPROpnbA) | Uninduced | <10 | ND |
| E. colic(pPROLar.A122) | Induced | <10 | ND |
a
ND, not determined.
b
Strain DH5α.
c
Strain DH5αPRO.