Table 1.
Protein Target | Antibody Reference | Fixation/Permeabilization | Antibody Dilution |
---|---|---|---|
Claudin-3 | 34–1700 (Invitrogen) | Ice-methanol 1′ | 1:40 |
Claudin-5 | 34–1600 (Invitrogen) | Ice-methanol 1′ | 1:100 |
Occludin | 71–1500 (Invitrogen) | Ice-methanol 1′ | 1:50 |
ZO-1 | 61–7300 (Invitrogen) | Paraformaldehyde 1% 10′; 3 PBS-CMF rinses of 5′; Triton X100 0.1% 10′ |
1:200 |