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. 2022 Sep 6;23(18):10235. doi: 10.3390/ijms231810235

Table 1.

Antibodies used for the immunostaining experiment. ZO-1: zonula occludens-1.

Protein Target Antibody Reference Fixation/Permeabilization Antibody Dilution
Claudin-3 34–1700 (Invitrogen) Ice-methanol 1′ 1:40
Claudin-5 34–1600 (Invitrogen) Ice-methanol 1′ 1:100
Occludin 71–1500 (Invitrogen) Ice-methanol 1′ 1:50
ZO-1 61–7300 (Invitrogen) Paraformaldehyde 1% 10′;
3 PBS-CMF rinses of 5′;
Triton X100 0.1% 10′
1:200