Skip to main content
. 2022 Sep 9;23(18):10445. doi: 10.3390/ijms231810445

Figure 7.

Figure 7

Effects of expressing edited and uneditable alleles of FgCSN12 on sexual reproduction. (A) Schematic drawing of FgCSN12 and its protein. The A-to-I RNA editing sites and efficiency are marked in red. The amino acid changes caused by A-to-I RNA editing are also marked in red. The PCI domain is labeled with a blue box. (B) Schematic drawing of the FgCSN12S406G and FgCSN12S406S mutants. The FgCSN12S406G mutant had the A1414GT to G1414GT mutation. The FgCSN12S406S mutant had the A1414GT to T1414CT mutation. (C) Three-day-old PDA cultures of the wild type (PH-1) and transformants of the Fgcsn12 mutant expressing the FgCSN12S406G (S406G) and FgCSN12S406S (S406S) alleles. Photographs were taken after 3 days of incubation. (D) Mating cultures of PH-1, S406G, and S406S were examined for perithecium formation (upper), ascospore discharge (middle), and asci with ascospores (lower) in 8 dpf perithecia. White arrows point to cirrhi. White bar = 1 mm; black bar = 20 μm.