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(A) OPLS-DA score plot for formula-fed samples (day 3 (green circles) vs. month 3 (yellow circles)), with A = 1 + 1 + 0, N = 48, R2X(cum) = 0.51, R2Y(cum) = 0.75, and Q2(cum) = 0.67 for pareto scaling and a 95% confidence level of p-value = 7.33 × 10−12. (B) S-line plot demonstrating the metabolites responsible for discrimination (1. citric acid, 2. urea, 3. dimethylamine, 4. taurine, 5. creatinine, 6. methyl succinate).
