The effect of 24 h treadmill exercise after MCAO on the expression of laminin and mi crovessel density. (A) The laminin expression was detected by immunofluorescence method in the brain of the rats in the Sham, MCAO, 1 day, 3 days, and 5 days in treadmill exercise 24 h after MCAO groups; bars: 50 μm; (B) Quantification of microvessel density as shown by laminin expression (% area stained) in the brain of the rats in the Sham, MCAO, 1 day, 3 days, and 5 days in treadmill exercise 24 h after MCAO group; (C) The laminin expression was detected by Western blot method in the brain of the rats in the Sham, MCAO, 1 day, 3 days and 5 days in treadmill exercise 24 h after MCAO groups. (*** p < 0.001 vs. Sham; # p < 0.01, ### p < 0.001 vs. MCAO, n = 3).