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. 2022 Sep 6;23(18):10257. doi: 10.3390/ijms231810257

Figure 3.

Figure 3

Detection of apoptosis/necrosis in hMSC after administration of modafinil (11.2 µg/mL) (A,B), atomoxetine (0.9 µg/mL) (C,D) and guanfacine (17.7 ng/mL) (E,F) for 24 h, 48 h and 72 h. Briefly, 10% DMSO was added to hMSC as a positive indicator of apoptosis/necrosis. The line diagram represents the luminescent signal of phosphatidylserine (PS) exposure and the fluorescent signal of secondary necrosis in hMSCs. Values are given as mean ± standard deviation to blank of three independent experiments with six biological replicates. Significance in difference between untreated, 10% DMSO-, modafinil-, atomoxetine- and guanfacine treated hMSC was determined by ANOVA followed by Tukey’s post hoc tests. * corresponds to a value of p < 0.05. *** corresponds to a value of p < 0.001.