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. 2022 Sep 9;23(18):10430. doi: 10.3390/ijms231810430

Figure 5.

Figure 5

Concentrations of IL-6 and TNF-α in culture medium of U-937. Cytokine levels were measured by sandwich ELISA using culture medium of U-937 cells treated with XOS or LPS for 24 h. LPS+EGCG—cells treated for 1 h with 1 μg/mL EGCG followed by addition of 100 μg/mL LPS and culture for 24 h; LPS—U-937 cells stimulated with 100 μg/mL LPS for 24 h; XOS—cells grown for 24 h in culture medium containing 200 μg/mL XOS; LPS+XOS—cells treated for 1 h with 200 μg/mL XOS followed by addition of 100 μg/mL LPS and culture for 24 h. Results are shown as mean ± SEM. All samples were assayed in triplicates. Statistical significance was determined in comparison with the control (cells grown for 24 h in complete DMEM). * p < 0.05; *** p < 0.001.