Figure 1.

Zfp281/ZNF281 expression increased during intestinal fibrosis progression. C57BL/6 male mice were subjected to cycles of 2.5% DSS (dextran sulfate sodium) for 2 weeks, followed by 1 week of recuperation to establish a mouse intestinal fibrosis model. DSS-treated mice were sacrificed at 6 (DSS W6; n = 8) and 9 weeks (DSS W9; n = 5) of treatment along with untreated controls (UNT W6 n = 8; UNT W9 n = 8). Zfp281 mRNA (A) and protein levels (B) were analyzed in distal colon specimens. Data are expressed as mean ± SEM. * = p-value ≤ 0.05; *** = p-value ≤ 0.001. Representative photomicrographs from 4 μm colonic mucosa sections incubated with an anti-Zfp281 antibody at 20× (left panel) and 40× (right panel) magnification. The box underlines the area of the section with the highest magnification. Scale bars correspond to 20 and 10 μm, respectively. Arrows indicate Zfp281-positive inflammatory and mesenchymal cells (C).