Figure 2.

The effects of beta-sitosterol on the transcript levels of three vital viral genes (ie1, DNA pol, and VP28). The experimental workflow is described in Figure S3. (A) Influences of different beta-sitosterol contents (6.25, 12.5, 25, 50, and 100 mg/kg) on the transcript levels of WSSV genes at 24 hpi. (B) The transcript levels of WSSV genes were significantly inhibited by beta-sitosterol at 24, 48, and 72 hpi. The 18S gene served as an internal reference. The data are shown as mean ± SD (n = 5). Asterisks indicate the significant difference between the experimental and control groups (* p < 0.05, ** p < 0.01).