Table 1.
SCNT summary for MC1R-modified pigs.
| #Donor Cells | Cell Genotypes 1 | #Recipient | Embryos Transferred 2 | Pregnant | Births | Robust at Weaning | Gender | Coat Colors |
|---|---|---|---|---|---|---|---|---|
| 2-24 | Hetero substitutions at 3 SNPs (V95M, L102P, and T243A), homo substitution at V164A | 19-937900 | 205 (164 cleaved) | + | 8 | 5 | Male | 2 dark black, 1 light black, and 5 red |
| 2-24 | Hetero substitutions at 3 SNPs (V95M, L102P, and T243A), homo substitution at V164A | 20-694608 | 206 (169 cleaved) | + | 3 | 1 | Male | 2 dark black and 1 red |
| 2-24 | Hetero substitutions at 3 SNPs (V95M, L102P, and T243A), homo substitution at V164A | 20-006896 | 238 (202 cleaved) | + | 6 | 2 | Male | 4 dark black and 2 red |
| 8-27 | Hetero substitutions at all 4 SNPs | 20-627908 | 207 (166 cleaved) | - | - | - | - | - |
| 8-27 | Hetero substitutions at all 4 SNPs | 20-688700 | 204 (181 cleaved) | + | 7 | 6 | Female | All dark black |
| 8-27 | Hetero substitutions at all 4 SNPs | 19-914004 | 206 (184 cleaved) | - | - | - | - | - |
| 2-24/8-27/8-18 3 | 2-24, hetero substitutions at 3 SNPs (V95M, L102P, and T243A), homo substitution at V164A; 8-27 and 8-18, hetero substitutions at all 4 SNPs | 19-940104 | 232 (cleaved unknown) | - | - | - | - | - |
| 2-24/8-27/8-18 | 2-24, hetero substitutions at 3 SNPs (V95M, L102P, and T243A), homo substitution at V164A; 8-27 and 8-18, hetero substitutions at all 4 SNPs | 18-419006 | 247 (cleaved unknown) | + | 6 | 5 | Female | 2 dark black, 1 light black, and 3 red |
| 2-24/8-27/8-18 | 2-24, hetero substitutions at 3 SNPs (V95M, L102P, and T243A), homo substitution at V164A; 8-27 and 8-18, hetero substitutions at all 4 SNPs | 20-006907 | 193 (all cleaved) | + | 0 | 0 | - | - |
1 Identified by presence of base substitutions in sequencing chromatograms of MC1R gene. 2 Cleaved and uncleaved embryos were all transferred into recipients. 3 Three cell colonies were mixed as nuclear donors for SCNT.