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. 2022 Sep 8;23(18):10356. doi: 10.3390/ijms231810356

Table 2.

Phenotypes and genotypes of cloned piglets.

#Recipient #Cloned Piglets Health Status Coat Color MC1R Genotype Mutation Types E Allele
AA95 AA102 gRNA Site 1 AA164 AA243
19-937900 1 Stillborn Dark black GTG/ATG CTG/CCG Homo sub GCG/GCG ACG/GCA Hetero substitutions at 3 sites, homo substitution at 1 site ED1/e
2 Stillborn Red GTG/GTG CTG/CTG Δ1/Δ1 GTG/GTG ACG/ACG Homo frameshift Indel e/e
3 Stillborn Red GTG/GTG CTG/CTG WT/Δ4 GTG/GTG ACG/ACG Hetero frameshift Indel e/e
4 Dead before weaning Red GTG/GTG CTG/CTG Δ1/Δ1 GTG/GTG ACG/ACG Homo frameshift Indel e/e
5 Survived until weaning Red GTG/GTG CTG/CTG Δ1/Δ1 GTG/GTG ACG/ACG Homo frameshift Indel e/e
6 Survived until weaning Red GTG/GTG CTG/CTG WT GTG/GTG ACG/ACG None e/e
7 Survived until weaning Dark black GTG/ATG CTG/CCG Homo sub GCG/GCG ACG/GCA Hetero substitutions at 3 sites, homo substitution at 1 site ED1/e
8 Survived until weaning Light black GTG/ATG CTG/CCG Homo sub GCG/GCG GCA/GCA Hetero substitutions at 2 sites, homo substitution at 2 sites ED1/E+
20-694608 9 Dead before weaning Dark black GTG/ATG CTG/CCG Homo sub GCG/GCG ACG/GCA Hetero substitutions at 3 sites, homo substitution at 1 site ED1/e
10 Dead before weaning Red GTG/GTG CTG/CTG +1/+1 GTG/GTG ACG/ACG Homo frameshift Indel e/e
11 Survived until weaning Dark black GTG/ATG CTG/CCG Homo sub GCG/GCG ACG/GCA Hetero substitutions at 3 sites, homo substitution at 1 site ED1/e
20-006896 12 Mummy Red GTG/GTG CTG/CTG Δ1/Δ1 GTG/GTG ACG/ACG Homo frameshift Indel e/e
13 Stillborn Dark black GTG/ATG CTG/CCG Homo sub GCG/GCG ACG/GCA Hetero substitutions at 3 sites, homo substitution at 1 site ED1/e
14 Stillborn Red GTG/GTG CTG/CTG Δ1/Δ1 GTG/GTG ACG/ACG Homo frameshift Indel e/e
15 Dead before weaning Dark black GTG/ATG CTG/CCG Homo sub GCG/GCG ACG/GCA Hetero substitutions at 3 sites, homo substitution at 1 site ED1/e
16 Survived until weaning Dark black GTG/ATG CTG/CCG Homo sub GCG/GCG ACG/GCA Hetero substitutions at 3 sites, homo substitution at 1 site ED1/e
17 Survived until weaning Dark black GTG/ATG CTG/CCG Homo sub GCG/GCG ACG/GCA Hetero substitutions at 3 sites, homo substitution at 1 site ED1/e
20-688700 18 Stillborn Dark black GTG/ATG CTG/CCG Homo sub GTG/GCG ACG/GCA Hetero substitutions at 4 sites ED1/e
19 Survived until weaning Dark black GTG/ATG CTG/CCG Homo sub GTG/GCG ACG/GCA Hetero substitutions at 4 sites ED1/e
20 Survived until weaning Dark black GTG/ATG CTG/CCG Homo sub GTG/GCG ACG/GCA Hetero substitutions at 4 sites ED1/e
21 Survived until weaning Dark black GTG/ATG CTG/CCG Homo sub GTG/GCG ACG/GCA Hetero substitutions at 4 sites ED1/e
22 Survived until weaning Dark black GTG/ATG CTG/CCG Homo sub GTG/GCG ACG/GCA Hetero substitutions at 4 sites ED1/e
23 Survived until weaning Dark black GTG/ATG CTG/CCG Homo sub GTG/GCG ACG/GCA Hetero substitutions at 4 sites ED1/e
24 Survived until weaning Dark black GTG/ATG CTG/CCG Homo sub GTG/GCG ACG/GCA Hetero substitutions at 4 sites ED1/e
18-419006 25 Dead before weaning Dark black GTG/ATG CTG/CCG Homo sub GTG/GCG ACG/GCA Hetero substitutions at 4 sites ED1/e
26 Survived until weaning Dark black GTG/ATG CTG/CCG Homo sub GTG/GCG ACG/GCA Hetero substitutions at 4 sites ED1/e
27 Survived until weaning Light black GTG/ATG CTG/CCG Hetero sub GTG/GCG ACG/GCA Hetero substitutions at 4 sites ED1/e
28 Survived until weaning Red GTG/GTG CTG/CTG WT GTG/GTG ACG/ACG None e/e
29 Survived until weaning Red GTG/GTG CTG/CTG WT GTG/GTG ACG/ACG None e/e
30 Survived until weaning Red GTG/GTG CTG/CTG WT GTG/GTG ACG/ACG None e/e

1 Mutation types at gRNA site: homo sub, homozygous substitution with synonymous mutation; Hetero sub, heterozygous substitution with synonymous mutation; WT, wild type; Δ1 or +1, Indel size.