Figure 2.

Overview of the methods available to create heritable edits in plants. (a) VIGE through sgRNA expression. Agrobacterium cells carrying viral vectors expressing sgRNAs are agroinfiltrated into the leaves of Cas9-overexpressing plants. The CRISPR/Cas9 complex induces targeted genome editing in individual plant cells, which can be used for regeneration. Alternatively, genome editing occurs as the virus moves systemically throughout the plant. Gene-edited Cas9 transgenic seeds are then obtained. After crossing with wild type (WT), Cas9 can be segregated out, and gene-edited DNA-free seeds are produced. (b) VIGE through Cas9 and sgRNA expression. Agrobacterium cells carrying viral vectors expressing Cas9 and sgRNAs are agroinfiltrated into the leaves of wild-type plants. The CRISPR/Cas9 complex induces targeted genome editing in individual plant cells, which can be used for regeneration. Gene-edited DNA-free seeds are produced by the regenerated plants.