Table 1.
Virus delivering CRISPR/Cas9 reagents or sgRNAs to plant cells for genome editing.
| Virus | Receptor Plants | Virus Insert Cargo | Target(s) | Tissue Culture | Mutations Heritable | Refs. |
|---|---|---|---|---|---|---|
| Tobacco rattle virus (TRV) | Cas9-expressing N. benthamiana | sgRNAs (+/−FT) | NbPDS, NbAG | No | Yes | [33] |
| TRV | Cas9-expressing Arabidopsis | sgRNAs | AtGL1, AtTT4 | No | No | [34] |
| TRV | Cas9-expressing N. benthamiana | sgRNAs | NbPDS3, NbPCNA | No | Yes | [35] |
| TRV | Cas9-expressing Arabidopsis | sgRNAs (+/−FT or tRNA) | AtFWA | No | Yes | [36] |
| TRV | Cas9-expressing N. benthamiana | sgRNAs | NbPDS3 | No | Yes | [40] |
| Potato virus X (PVX) | Cas9-expressing N. benthamiana | sgRNAs (+/−FT or tRNA) | NbXT2B, NbPDS, NbFT | Yes | Yes | [41] |
| PVX | N. benthamiana | Cas9 and sgRNAs | NbTOM1 | Yes | Yes | [37] |
| Barley yellow striate mosaic virus (BYSMV) | GFP-expressing N. benthamiana | Cas9 and sgRNAs | GFP | No | No | [38] |
| Sonchus yellow net rhabdovirus (SYNV) |
N. benthamiana (WT or GFP expressing) |
Cas9 and sgRNAs | GFP, NbPDS, NbRDR6, NbSGS3 | Yes | Yes | [39] |
| Pea early browning virus (PEBV) |
Cas9-expressing N. benthamiana | sgRNAs | NbPDS | No | No | [34] |
| Apple latent pherical virus (ALSV) | Cas9-expressing N. benthamiana soybean |
sgRNAs | NbPDS, EPSPS, GmGW2 | No | No | [36] |
| Barley stripe mosaic virus (BSMV) | Cas9-expressing wheat | sgRNAs (+/−FT or tRNA) | TaGW2, TaUPL3, TaGW7, TaQ | No | Yes | [42] |
| BSMV |
N. benthamiana; Cas9-expressing wheat |
sgRNAs (+/−FT or tRNA) | TaPDS, TaGW2, TaGASR7 | No | Yes | [43] |
| BSMV |
N. benthamiana; Cas9-expressing wheat |
sgRNAs (+/−FT or tRNA) | TaHRC | No | Yes | [44] |
| BSMV |
N. benthamiana (WT or GFP expressing); Cas9-expressing wheat/maize |
sgRNAs |
NbPDS, GFP GASR7, TMS5 |
Yes | Yes No |
[45] |
| Beet necrotic yellow vein virus (BNYVV) | Cas9-expressing N. benthamiana | sgRNAs | NbPDS3 | No | No | [46] |
| Cotton leaf crumple virus (CLCrV) |
Cas9-expressing Arabidopsis |
sgRNAs (+/−FT) | AtBRI1, AtGL2 | No | Yes | [47] |
| Foxtail mosaic virus (FoMV) |
Cas9-expressing N. benthamiana S.viridis, Maize | sgRNAs |
NbPDS SvCA2, ZmHKT1 |
No | No | [48] |
| FoMV | N.benthamiana | Cas9, sgRNAs (+/−P19) | NbPDS | No | No | [49] |
| Cabbage Leaf Curl virus (CaLCuV) |
Cas9-expressing N. benthamiana | sgRNAs | NbIspH, NbPDS | Yes | No | [50] |
| Tobacco etch virus (TEV) and PVX | N. benthamiana | Cas12a and sgRNAs, respectively | NbXT1, NbFT | No | No | [51] |
| Bean yellow dwarf virus (BeYDV) | Tobacco | Cas9 and sgRNAs | AtADH1 | Yes | Yes | [52] |
| BeYDV | Potato | Cas9 and sgRNAs | StALS1 | Yes | Yes | [53] |
| BeYDV | Tomato | Cas9 and sgRNAs | SlANT1 | Yes | Yes | [54] |
| BeYDV | Tomato | Cas9 and sgRNAs | SlCRTISO, SlPSY1 | Yes | Yes | [55] |
| Wheat dwarfing virus (WDV) | Wheat | Cas9 and sgRNAs | GFP, BFP | Yes | Yes | [56] |
| WDV | Rice | Cas9 and sgRNAs | GFP | Yes | Yes | [57] |
| Tobacco mosaic virus (TMV) | GFP-expressing N. benthamiana | Cas9 and sgRNAs | GFP, NbAGO1 | No | No | [58] |
| TMV | GFP-expressing N. benthamiana | Cas9 and sgRNAs | GFP | No | No | [59] |