Figure 13.

Gypenosides can reduce autophagy induced by H₂O₂. The results showed that compared with the blank control group, the expression of LC3II and BECN1 increased, and the expression of p62 decreased after H₂O₂ treatment. Compared with the H₂O₂ intervention group, the expression of LC3II and BECN1 decreased, while p62 increased in preintervention of gypenosides was further increased. These bands are quantified by densitometry and then normalized to the level of β-actin in the same sample. (b) The value obtained by dividing the band density of the transcription factor of LC3 II by the band density of LC3 I. On the other hand, (c) and (d) are expressed by the ratio of band density to β-actin band density. Three samples from each of the three GO donors were taken for the experiment. All detections are repeated 3 times, with 3 replicate wells each time (^∗ as P < 0.05 and ^∗∗ as P < 0.01; P < 0.05 means the difference is statistically significant, and P value is analyzed by one-way ANOVA).