Figure 6.

Effect of gypenosides on the production of apoptosis-related protein and autophagy-related protein mRNA in orbital fibroblasts of patients with GO. Intervention was carried out according to different groups. The extracted RNA was reverse transcribed by real-time fluorescence quantitative PCR. Preintervention of gypenosides could significantly inhibit the mRNA expression of caspase-3 and BAX in orbital fibroblasts of GO and non-GO patients. Three samples from each of the three GO and non-GO donors were taken for the experiment. All detections are repeated 3 times, with 3 replicate wells each time (^∗ as P < 0.05 and ^∗∗ as P < 0.01; P < 0.05 means the difference is statistically significant, and P value is analyzed by two-way ANOVA).