FIG. 3.

SDS-PAGE (18% polyacrylamide) analysis showing the genetic complementation of an lpcC mutant of R. leguminosarum bv. viciae (Rle) by the lpsB gene of S. meliloti. Lanes: Rle 3855, wild-type strain; Rle RSKnH, lpcC mutant; Rle RSKnH (pPN120), lpcC mutant carrying a plasmid copy of the wild-type lpcC gene; Rle RSKnH (pJBlpsSme), lpcC mutant carrying a plasmid copy of the wild-type lpsB gene of S. meliloti (Table 1). Each well contained the total LPS extracted from about 10 mg (wet weight) of cells of the indicated bacterial strain, using EDTA and polymyxin B as described in Materials and Methods. The positions of LPS components in the gel are indicated to the left.