TABLE 1.
Strains used and constructed for this work
| Strain | Relevant characteristics | Source and/or reference |
|---|---|---|
| Agrobacterim tumefaciens C58 | Wild type | A. G. Matthysse |
| Bradyrhizobium japonicum USDA 110 | Wild type, symbiont of Glycine max | P. van Berkum |
| Escherichia coli DH5α | recA1, ΔlacU169 80dlacZΔM15; host strain used in cloning experiments | Bethesda Research Laboratories |
| E. coli XL1-Blue | Host strain of the nested-deletion clones generated by exonuclease III for DNA sequencing | Bullock et al. (10) |
| E. coli S17-1 | MM294, RP4-2-Tc::Mu-Km::Tn7 chromosomally integrated | Simon et al. (54) |
| Mesorhizobium ciceri USDA 3383 | Wild type, symbiont of Cicer arietinum L. | P. van Berkum |
| M. tianshanense USDA 3592 | Wild type, symbiont of Glycirrhiza pallidiflora | P. van Berkum |
| Rhizobium etli CFN42, USDA 9032 | Wild type, symbiont of Phaseolus vulgaris | E. Martínez |
| R. galegae USDA 4128 | Wild type, symbiont of Galega orientalis | P. van Berkum |
| R. hainanensis USDA 3588 | Wild type, symbiont of Desmodium sinuatum | P. van Berkum |
| R. huatlense USDA 4900 | Wild type, symbiont of Sesbania herbaceae | P. van Berkum |
| R. leguminosarum bv. viciae VF39 | Wild type, symbiont of Pisum sativum | U. Priefer |
| R. leguminosarum bv. viciae 3855 | Wild type, symbiont of P. sativum, Smr derivative of wild-type strain 128C53 | C. Ronson (28) |
| R. leguminosarum bv. viciae RSKnH | R. leguminosarum bv. viciae 3855 lpcC::nptII | C. Ronson (28) |
| R. leguminosarum bv. trifolii ANU843 | Wild type, symbiont of Trifolium spp. | P. van Berkum |
| R. mongolense USDA 1844 | Wild type, symbiont of Medicago ruthenica | P. van Berkum |
| Rhizobium sp. strain GRH2 | Wild type, isolated from Acacia cyanophylla, nodulates herbaceous legumes such as Phaseolus and Trifolium spp. | N. Toro |
| Rhizobium sp. strain LPU83 | Wild type, nodulates M. sativa, P. vulgaris, and Leucaena leucocephala, closely related to Rhizobium sp. strain Or191 | Del Papa et al. (17) |
| Rhizobium sp. strain NGR234 | Wild type, isolated from root nodules of Lablab purpureus, nodulates several legumes | W. J. Broughton |
| Rhizobium sp. strain Or191 | Wild type, nodulates M. sativa, P. vulgaris, and L. leucocephala | P. van Berkum |
| R. tropici CIAT 299 (type 2A) | Wild type, symbiont of P. vulgaris | M. Aguilar |
| R. tropici CIAT 899 (type 2B), USDA 9030 | Wild type, symbiont of P. vulgaris | P. van Berkum |
| Sinorhizobium sp. strain BR816 | Wild type, symbiont of P. vulgaris | M. Aguilar |
| Sinorhizobium fredii HH103 | Wild type, symbiont of G. max | M. Megias |
| S. fredii USDA 191 | Wild type, symbiont of G. max | S. Pueppke |
| S. fredii USDA 257 | Wild type, symbiont of G. max | S. Pueppke |
| S. medicae USDA 1037 | Wild type, symbiont of M. sativa | P. van Berkum |
| S. meliloti 2011 | Symbiont of M. sativa, Smr derivative of strain SU-47 | Casse et al. (12) |
| S. meliloti 6963 | S. meliloti 2011 lpsB::Tn5-6963 Nmr Smr | Lagares et al. (34) |
| S. meliloti 7555 | S. meliloti SU47 lpsC::Tn5 Nmr Smr | Clover et al. (15) |
| S. meliloti 20-B+ | S. meliloti 2011 lpsB::lacZ-Gm (sense lacZ fusion at KpnI site in lpsB) Smr Gmr | This work |
| S. meliloti 20-B | S. meliloti 2011 lpsB::lacZ-Gm (antisense lacZ fusion at KpnI site in lpsB Smr Gmr | This work |
| S. meliloti 20-C | S. meliloti 2011 lpsC::pK18mob, lacZ promoter of vector reads downstream of interrupted coding sequence, Smr Nmr | This work |
| S. meliloti 20-D | S. meliloti 2011 lpsD::pK18mob, lacZ promoter of vector reads downstream of interrupted coding sequence, Smr Nmr | This work |
| S. meliloti 20-E | S. meliloti 2011 lpsE::pK18mob, lacZ promoter of vector reads downstream of interrupted coding sequence, Smr Nmr | This work |
| S. saheli USDA 4893 | Wild type, symbiont of Sesbania cannabina | P. van Berkum |
| S. teranga USDA 4894 | Wild type, symbiont of Sesbania laeta | P. van Berkum |