Figure 7.

The role of TLR4 in SAA1-induced monocyte and platelet adhesion to endothelial cells. HUVECs were treated with SAA1 or SAA1+TAK-242 for 24 h, with the untreated HUVECs as the control. These HUVECs were used for coculture with THP-1 cells/platelets preincubated with calcein AM for 15 min. (a) Six hours later, the number of THP-1 cells adhering to endothelial cells was observed by fluorescence microscopy. (b) After coculture with platelets for 1 h, the nuclei of HUVECs were stained by DAPI. Scale bar, 25 μm and 50 μm. Data represent the mean ± SEM; n = 6 (^∗∗∗P < 0.001).