Figure 3.

UCA1 increases trophoblast viability under hypoxic conditions. (a–d) EdU (a–b) and CCK8 (c–d) assays of cells with UCA1 overexpression and knockdown. (e–g) Transwell assays of cells with UCA1 overexpression and knockdown. HTR8/SVneo and JEG-3 cells were transfected with UCA1 overexpression and control lentiviruses. JEG-3 cells were transfected with Smart Silencer and negative control. Cells were incubated with 250 μM CoCl₂ or PBS as a negative control. ^∗∗P < 0.01, ^∗∗∗P < 0.001, and ^∗∗∗∗P < 0.0001.