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. 2022 Sep 21;221(10):e202111076. doi: 10.1083/jcb.202111076

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© 2022 Liu et al.

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Figure S4. — N-terminal 15 amino acids are dispensable for ARL3^Q70L to maintain the ciliary content of the BBSome and PLD and ARL3 does not affect BBSome integrity in cilia. (A) Immunoblots of WC samples and cilia of CC-5325, arl3-282, arl3-282; ARL3ΔN15Q70L:HA:YFP-TG cells probed for the BBSome subunits BBS1, BBS4, BBS5, BBS7, BBS8, and PLD. α-tubulin and acetylated (Ac)-tubulin were used to adjust the loading of WC samples and cilia, respectively. (B) Immunoblots of sucrose density gradients of CC-5325, arl3-282, arl3-282; ARL3:HA:YFP-TG, arl3-282; ARL3Q70L:HA:YFP-TG, and arl3-282; ARL3T30N:HA:YFP-TG cilia probed for BBS1, BBS4, BBS5, BBS7, and BBS8. These BBSome proteins co-sedimented completely. Source data are available for this figure: SourceData FS4.