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. 2022 Sep 21;221(10):e202111076. doi: 10.1083/jcb.202111076

Table 1.

List of primary antibodies used in this study

Name IB IS Host Reference or source
Anti-ARL3 1:250 N/A Rb This study
Anti-CEP290 1:250 1:50 Rb This study
Anti-IFT22 1:1,000 N/A Rb (Xue et al., 2020)
Anti-IFT38 1:1,000 N/A Rb (Sun et al., 2021)
Anti-IFT46 N/A 1:100 Rb (Dong et al., 2017a)
Anti-IFT57 1:500 N/A Rb (Dong et al., 2017a)
Anti-IFT43 1:500 N/A Rb (Zhu et al., 2017)
Anti-IFT70 1:1,000 N/A Rb (Dong et al., 2017a)
Anti-IFT81 N/A 1:200 Mo (Fan et al., 2010)
Anti-IFT139 1:1,000 N/A Rb (Dong et al., 2017a)
Anti-BBS1 1:1,000 N/A Rb (Xue et al., 2020)
Anti-BBS4 1:500 N/A Rb (Liu et al., 2021)
Anti-BBS5 1:1,000 N/A Rb (Xue et al., 2020)
Anti-BBS7 1:500 N/A Rb (Liu et al., 2021)
Anti-BBS8 1:500 1:50 Rb (Sun et al., 2021)
Anti-PLD 1:1,000 N/A Rb (Liu et al., 2021)
Anti-α-tubulin (B512) 1:10,000 N/A Mo Sigma-Aldrich (T5168)
Anti-acetylated-tubulin (6-11B-1) 1:10,000 N/A Mo Sigma-Aldrich (T7451)
Anti-YFP (7.1 and 13.1) 1:1,000 1:50 Mo Roche (11814460001)
Anti-HA (3F10) 1:1,000 1:50 Rt Roche (11867423001)
HRP-conjugated goat anti-rabbit IgG 1:10,000 N/A Gt The Jackson Lab. (111035003)
HRP-conjugated goat anti-mouse IgG 1:10,000 N/A Gt The Jackson Lab. (115035003)
HRP-conjugated goat anti-rat IgG 1:10,000 N/A Gt The Jackson Lab. (112035003)
Alexa-Fluor 594-conjugated goat anti-rabbit IgG N/A 1:400 Gt Molecular probes (A11012)
Alexa-Flour 488-conjugated goat anti-mouse IgG N/A 1:400 Gt Molecular probes (A10680)
Alexa-Flour 488-conjugated goat anti-rat IgG N/A 1:400 Gt Molecular probes (A11006)

Rb, rabbit; Mo, mouse; Rt, rat; Gt, goat; IB, immunoblotting; IS, immunostaining.