Table 3.

List of primers used in this study

Name	Nucleotide sequence
Primers used to amplify the aphVIII gene insertion in ARL3 genomic DNA
gARL3-FOR	5′-CCT​CTA​GAA​CAT​CAC​CAC​TAT​CAC​GC-3′
gARL3-REV	5′-CCG​AAT​TCC​CAA​AGC​TCC​GAA​GCC​AC-3′
Primers used to clone target genes
gARL3-FOR1	5′-CCT​CTA​GAA​GCT​CCG​ACA​TGA​GCG​CC-3′
gARL3-REV1	5′-CCG​AAT​TCC​TTG​ACC​TGC​TTC​ATC​ATC-3′
gARL3ΔN15-FOR	5′-CCG​GAT​CCG​TGA​CAT​GTG​CTC​GCA​GC-3′
gARL3ΔN15-REV	5′-CCG​GAT​CCC​TGT​GGC​GAG​CAC​ACC​AG-3′
gIFT38-FOR	5′-CCG​GAT​CCA​TTT​AGC​CCC​AAA​TTA​TG-3′
gIFT38-REV	5′-CCG​AAT​TCG​AAG​TCA​TTG​TCA​TCC​TC-3′
gIFT43-FOR	5′-ACG​CGG​CCG​CAT​GGT​GTC​CTA​CCT​GGG​C-3′
gIFT43-REV	5′-GTG​AAT​TCC​AGC​TTG​ATG​GGC​ATG​G-3′
gBBS8-FOR	5′-GGA​CTA​GTT​GCA​GCA​GCA​AGT​AAT​GCA​AC-3′
gBBS8-REV	5′-GGA​ATT​CCA​GCA​TAG​TGA​AAT​GGG​CC-3′
cARL3-FOR1	5′-GGC​CAT​GGA​TGG​GCC​TCT​TGT​CGC​TG-3′
cARL3-REV1	5′-CGG​CGG​CCG​CCT​TGA​CCT​GCT​TCA​TC-3′
cARL3ΔN15-FOR	5′-CCC​CAT​GGA​TGG​CCC​GCA​TCC​TGG​TC-3′
cBBS1-FOR	5′-CCG​GAT​CCA​TGC​TGC​CAT​CAG​TCA​AG-3′
cBBS1-REV	5′-CCA​AGC​TTT​TAC​TCC​ACC​TCC​TCC​GG-3′
cBBS2-FOR	5′-CCG​GAT​CCA​TGC​TCG​TGC​CGG​CCT​TC-3′
cBBS2-REV	5′-CCA​AGC​TTT​CAC​ACC​GGC​CCG​TCG​CC-3′
cBBS4-FOR	5′-CAG​CAA​ATG​GGT​CGG​GAT​CCA​TGT​CGT​CAT​TAG​CGC​AG-3′
cBBS4-REV	5′-TCG​ACG​GAG​CTC​GAA​TTC​TTA​TCA​CAT​GCC​CAG​CAG​CT-3′
cBBS5-FOR	5′-GAC​AGC​AAA​TGG​GTC​GGG​ATC​CAT​GGC​TGA​CCT​CTT​TG-3′
cBBS5-REV	5′-CGA​CGG​AGC​TCG​AAT​TCT​TAT​CAC​AGC​ACG​CTC​CAC​AG-3′
cBBS7-FOR	5′-GGA​CAG​CAA​ATG​GGT​CGG​GAT​CCA​TGG​AGC​TGG​AAC​TAT​TTC-3′
cBBS7-REV	5′-GTC​GAC​GGA​GCT​CGA​ATT​CTT​ACT​ACC​GCT​GCC​CCA​GCA​G-3′
cBBS8-FOR	5′-GGA​CAG​CAA​ATG​GGT​CGG​GAT​CCA​TGC​AGC​AGC​CAC​AGC​AAG-3′
cBBS8-REV	5′-GTC​GAC​GGA​GCT​CGA​ATT​CTT​ATC​ACA​GCA​TAG​TGA​AAT​G-3′
Primers used to do site-directed mutagenesis
ARL3Q70L-FOR	5′-GGA​CAT​TGG​CGG​CCT​GAA​GTC​CAT-3′
ARL3Q70L-REV	5′-AGG​CCG​CCA​ATG​TCC​CAA​ATT​TTC-3′
ARL3T30N-FOR	5′-ATA​ACG​CTG​GTA​AAA​ACA​CCA​TCC​TG-3′
ARL3T30N-REV	5′-TTT​TTA​CCA​GCG​TTA​TCC​AGT​CCC​A-3′