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. 2022 Aug 25;4(9):1109–1118. doi: 10.1038/s42255-022-00615-8

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Extended Data Fig. 6 — a, Validation of immunofluorescence staining of LTL, VCAM1 and KIM1 on the measured area of post-MSI slice compared to consecutive slice without MALDI matrix. Scale bar = 100 µm. b, Immunofluorescence staining of LTL, VCAM1 and KIM1 on bIRI kidney, same as the image shown in Fig. 4b. Scale bar = 200 µm.