Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 Aug 25;4(9):1109–1118. doi: 10.1038/s42255-022-00615-8

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Extended Data Fig. 7 — a, MECA32 staining of different vessels in mouse kidney to show specific capillary endothelial cell staining (Scale bar = 5 µm). Arrows depict the vessels with negative MECA32 staining. b, Metabolic heterogeneity within cortical cells in kidney, visualized in a two-dimensional UMAP plot of combined MALDI-MSI data and immunofluorescence staining at 5 × 5 µm² pixel-size. Glomerular endothelial cells (gREC) and peritubular endothelial cells (cREC) were identified using the post-MALDI-MSI anti-MECA32 antibody staining and podocytes (Podo) with anti-NPHS1 antibody staining. Proximal tubules (PT), distal tubules (DT) and collecting ducts (CD) were identified based on their respective lipid profiles obtained from measurement shown in Extended Data Fig. 1b. c, Immunofluorescent (MECA32 and NPHS1, middle panel; scale bar = 40 µm) staining on post-MALDI-MSI tissue to determine gREC, cREC and podocyte distribution (left panel). Pixel colors used are similar to the given UMAP cell-cluster colors in A. Inset view of detailed glomerular area (right panels, scale bar = 5 µm). d, MECA32 expression in the observed cell types. e, Dynamic metabolic measurements using U-¹³C₆-glucose. Graphs show the ¹³C enrichment of isotopologues of glucose, glycerol 3-phosphate (G3P), 3-phosphoglycerate (3PG), lactate and glutamate over time in gREC and cREC. Two tailed paired t-test was performed (n = 3). Spatial UMAP shows the metabolic cellular architecture of the tissue (arrows depict cREC and gREC). Pixel colors used are similar to the given UMAP cell-cluster colors in B. Scale bar = 200 µm.

Source data