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. 2022 Aug 25;4(9):1109–1118. doi: 10.1038/s42255-022-00615-8

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Extended Data Fig. 1 — a, Lipid species distribution in a mouse kidney tissue sample as recorded by MALDI-MSI at 20 × 20 μm² pixel-size. b, Dot plot displaying lipid expression of cluster-enriched signatures. c, Distribution of different renal cell clusters on tissue as identified in Fig. 2a. d, Immunofluorescent staining (Lotus Tetragonolobus Lectin (LTL, green), E-cadherin (CDH1, red) and BS1-lectin (gray)) after MALDI-MSI measurement. e, Nephron anatomy showing the identification of renal cell types based on immunofluorescent staining. f, Molecular histology of kidneys generated from integrated three-dimensional UMAP analysis of different datasets based on lipid profiles showing the specificity and stability of lipids profiles after introducing ¹³C-labeled nutrients for 2 hours. All scale bars = 500 µm. Abbreviations: PT_S1/S2, cortical proximal tubular segments 1/2; PT_S3, outer stripe of outer medulla proximal tubular segment; DT, distal tubule; CDLH, collecting duct and loop of Henle; IM, inner medulla; ISOM, inner stripe of outer medulla; OSOM, outer stripe of outer medulla; PUL, pelvic urothelial lining.