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. 2022 Sep 5;28(9):1813–1822. doi: 10.1038/s41591-022-01956-3

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Extended Data Fig. 2 — Cell product differentiation into astrocytes. Representative image and quantification of double immunofluorescence for (a, b) CNS10-NPC-GDNF labeled with Stem101 (nuclear specific, green) and human nestin, a neural progenitor marker, and with (c, d) GFAP, an astrocyte marker (red). (e) Double immunofluorescence shows that many cells are positive for human nestin and GFAP. (f) Confocal microscopy images show that CNS10-NPC-GDNF labeled with Stem 121 (cytoplasmic specific, red) co-localized with mature astrocyte markers aquaporin 4 (AQP4) and Glast (green). Nuclei counterstained with DAPI (blue). White arrows indicate CNS10-NPC-GDNF co-expressing the markers. Sample size n = 15 biologically independent animals for each dose and n = 10 for vehicle. Error bars ± s.e.m. Scale bars: (a, c) 10 µm; (e) = 75 µm; (f low mag) 10 µm; (f high mag) 5 µm.