Extended Data Fig. 2. Nnat+/-p-Heavy morphs exhibit accelerated post-weaning growth kinetics associated with hyperinsulinemia.
a, Birth weight was measured for Nnat+/-p male newborns. Each group had at least 22 animals. Data are presented as mean ± SEM. b, Fat (top) and lean (middle) mass deposition between 4 to 7 weeks were plotted for male Nnat+/-p colony. Daily food intake (button) was measured and the average daily food intake were calculated per week between 4 to 7 weeks for male Nnat+/-p colony. All data were plotted as mean ± SEM. Adjusted p-values by one-sided Tukey’s multiple comparisons test. Top panel *** p < 0.0001; middle panel * p = 0.016, *** p < 0.0001; lower panel * p = 0.0019. c, Daily food intake normalized to body weight (top) and body weight deposition normalized to average daily food intake per week (button) between 4 to 7 weeks were plotted for male Nnat+/-p colony. All data were plotted as mean ± SEM. Adjusted p-values by one-sided Tukey’s multiple comparisons test. Lower panel * p = 0.0104, ** p = 0.002, *** p < 0.0001. d, Circulating growth factors (insulin-like growth factor 1, IGF1; growth hormone, GH; and insulin, INS) were detected in plasma of Nnat+/p males and WT littermates at 4 and 6 weeks of age. Each group had at least 3 animals. All data were plotted as mean ± SEM. Adjusted p-values by one-sided Tukey’s multiple comparisons test. ** p = 0.0001, *** p < 0.0001.