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. 2022 Aug 29;2(9):100280. doi: 10.1016/j.crmeth.2022.100280

Figure 2.

Figure 2

Direct contact co-culture is required for non-myocyte regulation of iPSC-CM electrophysiology

(A) Experimental outline showing combined encapsulation of all cells (direct contact) or separation of each cell type (indirect contact).

(B) Quantification of iPSC-CM spontaneous beating rate.

(C) iPSC-CM calcium transient amplitude.

(D) iPSC-CM calcium transient time to peak.

(E) iPSC-CM calcium transient time to 80% decay.

Data are shown as mean ± SEM. Each datapoint represents 1 dish, containing average of 3 cells, 3 beats/cell, N = 3.